Fig. 7
From: miR-3065-3p promotes stemness and metastasis by targeting CRLF1 in colorectal cancer
CRLF1 inhibits tumorigenesis and metastasis of colorectal cancer in vivo. a, b A mouse model of colorectal cancer with five mice in each group was established via orthotopic implantation of HCT116 cells overexpressing CRLF1 or vector control (MCS). Images showing the tumor size (a) and statistical results of tumor volume (b) are shown. A liver metastasis mouse model of colorectal cancer with five mice in each group was established via spleen injection using CRLF1-overexpressing HCT116 or control cells. c, d Metastatic nodules of the liver (c) and representative images of HE-stained metastatic lesions by (d) are shown. e, f Western blot (e) and immunohistochemical (f) analysis of NANOG, OCT4, and SOX2 expression in tumor tissue from xenograft mice. ‘1–4’ represented the different four mice in the same group. Statistical results are shown in the right panel. The data shown represent the mean values ± SEM of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001