Fig. 5
From: miR-3065-3p promotes stemness and metastasis by targeting CRLF1 in colorectal cancer
CRLF1 suppresses the stemness of colorectal cancer cells in vitro. a, b RT-qPCR (a) and western blot (b) analysis of CRLF1 overexpression in HCT116 cells. c Representative images of the wound healing assay and statistical results of the migration area are shown. d Transwell assay of the migration of CRLF1-overexpressing HCT116 cells and vector controls (MCS)-expressing cells and the statistical results. e, f The mRNA (e) and protein (f) levels of NANOG, OCT4, and SOX2 in CRLF1-overexpressing HCT116 cells were analyzed by RT-qPCR and western blotting. g Representative images and statistical results of sphere formation assays. h Flow cytometric analysis of ALDH activity in CRLF1-overexpressing HCT116 cells. Statistical results are shown in the right panel. The data shown represent the mean values ± SEM of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001