Fig. 2
From: miR-3065-3p promotes stemness and metastasis by targeting CRLF1 in colorectal cancer
miR-3065-3p promotes the stemness of colorectal cancer cells in vitro. a RT-qPCR analysis of miR-3065-3p overexpression in HCT116 and RKO cells. b Representative images of the wound healing assay and statistical results of the migration area are shown. c Transwell assay of the migration of miR-3065-3p-overexpressing HCT116 or RKO cells compared and negative control (NC)-expressing cells and the statistical results. d, e The mRNA (d) and protein (e) levels of NANOG, OCT4, and SOX2 in miR-3065-3p-overexpressing HCT116 or RKO cells were analyzed by RT-qPCR and western blotting. f Representative images and statistical results of sphere formation assays. Statistical results are shown in the right panel. g Flow cytometric analysis of ALDH activity in HCT116 and RKO cells transfected with miR-3065-3p mimics or negative control (NC). Statistical results are shown in the right panel. The data shown represent the mean values ± SEM of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001