Fig. 5

MiR-144-3p inhibits gastric cancer progression and stemness. a CCK-8 assay to detect cell viability of HGC-27 transfected with miR-144-3p mimics or negative control (NC). b, c Clone formation assay of HGC-27 transfected with miR-144-3p mimics or NC. The clone number of HGC-27 was counted. d Cell cycle was detected by flow cytometry in HGC-27. e Cell proliferation of SGC-7901 was detected by CCK-8 after transfection with miR-144-3p inhibitor or NC. f, g Clone formation assay of SGC-7901 transfected with miR-144-3p inhibitor or NC. The clone number of SGC-7901 was counted. h Cell cycle was detected by flow cytometry in SGC-7901. i, j Transwell assay to detect the migration and invasion of HGC-27 treated with mimics or NC. The migrated and invaded cells were counted. k, l Transwell assay to detect migration and invasion of SGC-7901 treated with inhibitor or NC. The migrated and invaded cells were counted. m, n Tumorsphere formation assay of gastric cancer stem cells (GCSCs) treated with miR-144-3p mimic or inhibitor and corresponding NC. The number of tumorsphere > 50 μm in per field was counted. o, p The CD44 expression of GCSCs treated with miR-144-3p mimic or inhibitor was detected by flow cytometry. q The related protein expression after miR-144-3p intervention in HGC-27 and SGC-7901. (Scale bar = 100 μm, *p < 0.05, **p < 0.01, ***p < 0.001)