Fig. 5

FAM83A promotes EMT through Wnt/β-catenin signaling pathway and β-catenin regulated FAM83A expression in HNSCC cells. A Expression of nuclear and cytoplasmic FAM83A and β-catenin protein in CAL27 and HN6 cells after FAM83A silencing. B The expressions of nuclear and cytoplasmic FAM83A and β-catenin protein in CAL27 and HN6 cells after FAM83A overexpressed. C Immunofluorescence assays of β-catenin in CAL27 and HN6 cells after FAM83A silencing(400 ×). D Immunofluorescence assays of β-catenin in CAL27 and HN6 cells after FAM83A overexpressed (400 ×). E Protein levels of β-catenin, FAM83A, E-cadherin, N-cadherin, Vimentin, and Snail were determined by western blotting in CAL27 and HN6 cells after being treated with XAV-939. F Protein levels of β-catenin and FAM83A were determined by western blotting in CAL27 and HN6 cells after si-β-catenin transfection and plasmid transfection respectively