Fig. 7

The Wnt/β-catenin pathway is involved in CENPA-mediated proliferation and metastasis. A A498 and Caki-1 cells with CENPA overexpression were treated with Si-CTNNB1 for 48 h. Then, expression of CTNNB1 was measured by western blotting. B, C A498 and Caki-1 cells with CENPA overexpression or knockdown were treated with Wnt-pathway inhibitor XAV-939 (10 μmol/L) or agonist CHIR-99012 (10 μmol/L) for 24 h. Then, western blotting determined that the drugs were effective to Wnt pathway. D–F Cell viability was assessed in A498 and Caki-1 cells with CENPA overexpression and Wnt pathway inhibition/excitation via CCK-8 assays (n = 4 per group). G, H Caki-1 cells with overexpression or knockdown of CENPA were treated with Si-CTNNB1 for 48 h or CHIR-99021 (10 μmol/L) for 24 h as indicated and subjected to migration assay and invasion assay (magnification: 200×, n = 3 per group). NC: negative control; n.s: not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Error bars indicate mean ± SD