Fig. 6

CENPA accelerated the cell cycle and activated the Wnt signaling pathway. A The GSEA results of CENPA using TCGA-KIRC expression dataset. 533 tumoral samples was divided into two groups based on CENPA level. Genes expression patterns of two groups was different in cell cycles and Wnt pathway. B The expression of WNT5A is positively correlated to the expression of CENPA in TCGA-KIRC ccRCC tissues. C, D The expression of cyclin D1(CCND1) and β-catenin (CTNNB1) were down-regulated or upregulated in ccRCC cells with knockdown or overexpression of CENPA. E Western blot revealed the expression of CTNNB1 in the cytoplasm and nucleus. β-actin and Lamin B1 were used as internal references in the cytoplasm and nucleus, respectively. F–G Cell cycle distribution was analyzed by PI staining in Caki-1 cells after transfection by Si-CENPA or CENPA plasmid for 48 h. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Error bars indicate mean ± SD