Fig. 1

Preoperative MRI and ¹⁸F-FDG-PET imaging in the donor patient and corresponding preclinical MRI and ¹⁸F-FDG-PET imaging in the developed orthotopic O-PDX mouse model. Upper panel: Axial-oblique MRI sequences (A–C) displaying an irregularly shaped large uterine primary tumor invading > 50% the myometrial wall (white arrows) and enlarged pelvic left sided lymph node (yellow arrows), all exhibiting restricted diffusion (B and C). On PET-CT high ¹⁸F-FDG avidity is seen both in the primary tumor (white arrows) and in the bilateral pelvic lymph nodes (yellow arrows) (axial (D) and coronal (E) planes). Lower panel: Coronal MRI of a representative mouse tumor (white arrows) in the left uterine horn (F–H) 3 weeks after implantation displaying characteristic hyperintensity on T2 (F) and high b-value image (DWI) (G) and corresponding hypointense on the ADC map (H) indicating restricted diffusion. Abdominal axial (I) and maximum intensity projection (MIP) images from ¹⁸F-FDG-PET-CT (J) depict a highly ¹⁸F-FDG- avid uterine tumor in the same mouse, 2 days after the MRI examination. b bladder, k kidney, ADC apparent diffusion coefficient, DWI diffusion-weighted MRI, ¹⁸F-FDG fluorodeoxyglucose, O-PDX organoid-based patient-derived xenograft, SUV standardized uptake value