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Table 2 The information of antibodies used for western blot, immunohistochemistry, and flow cytometry

From: Niraparib exhibits a synergistic anti-tumor effect with PD-L1 blockade by inducing an immune response in ovarian cancer

Antibodies

Reactivity

Dilution

Catalogue

Application

GAPDH

Human

1:1000

Cell Signal Technology, #5174

WB

PARP1

Human

1:1000

Abcam, #ab191217

WB; IHC

PD-L1

Human

1:200;2ug/ml

Abcam, #ab205921

WB; IHC

STING

Human

1:1000

Cell Signal Technology, #13,647

WB

p-STING_S366

Human

1:1000

Cell Signal Technology, #50,907

WB

TBK1

Human

1:1000

Cell Signal Technology, #3504

WB

p-TBK1_S172

Human

1:1000

Cell Signal Technology, #5483

WB

IRF3

Human

1:1000

Cell Signal Technology, #11,904

WB

p-IRF3_S396

Human

1:1000

Cell Signal Technology, #29,047

WB

CD8

Human

1:250

Abcam, ab93278

IHC

IFN-γ

Human

1:50

Novus, #JM10-10

IHC

CD3 and isotype

Human

5 μl of antibody per test

Elabscience, E-AB-F1001E

Flow cytometry

CD4 and isotype

Human

5 μl of antibody per test

Elabscience, E-AB-F1109I

Flow cytometry

CD8 and isotype

Human

5 μl of antibody per test

Elabscience, E-AB-F1110D

Flow cytometry

PD-L1 and isotype

Human

5 μl of antibody per test

Elabscience, E-AB-F1133E

Flow cytometry

IFN-γ and isotype

Human

5 μl of antibody per test

Thermo Fisher Scientific, 48–7319-41

Flow cytometry

CD3 and isotype

Mouse

5 μl of antibody per test 

Elabscience, E-AB-F1013J

Flow cytometry

CD4 and isotype

Mouse

5 μl of antibody per test

Elabscience, E-AB-F1097C

Flow cytometry

CD8 and isotype

Mouse

5 μl of antibody per test

Elabscience, E-AB-F1104D

Flow cytometry

IFN-γ and isotype

Mouse

5 μl of antibody per test

Elabscience, E-AB-F1101E

Flow cytometry