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Table 2 The information of antibodies used for western blot, immunohistochemistry, and flow cytometry

From: Niraparib exhibits a synergistic anti-tumor effect with PD-L1 blockade by inducing an immune response in ovarian cancer

Antibodies Reactivity Dilution Catalogue Application
GAPDH Human 1:1000 Cell Signal Technology, #5174 WB
PARP1 Human 1:1000 Abcam, #ab191217 WB; IHC
PD-L1 Human 1:200;2ug/ml Abcam, #ab205921 WB; IHC
STING Human 1:1000 Cell Signal Technology, #13,647 WB
p-STING_S366 Human 1:1000 Cell Signal Technology, #50,907 WB
TBK1 Human 1:1000 Cell Signal Technology, #3504 WB
p-TBK1_S172 Human 1:1000 Cell Signal Technology, #5483 WB
IRF3 Human 1:1000 Cell Signal Technology, #11,904 WB
p-IRF3_S396 Human 1:1000 Cell Signal Technology, #29,047 WB
CD8 Human 1:250 Abcam, ab93278 IHC
IFN-γ Human 1:50 Novus, #JM10-10 IHC
CD3 and isotype Human 5 μl of antibody per test Elabscience, E-AB-F1001E Flow cytometry
CD4 and isotype Human 5 μl of antibody per test Elabscience, E-AB-F1109I Flow cytometry
CD8 and isotype Human 5 μl of antibody per test Elabscience, E-AB-F1110D Flow cytometry
PD-L1 and isotype Human 5 μl of antibody per test Elabscience, E-AB-F1133E Flow cytometry
IFN-γ and isotype Human 5 μl of antibody per test Thermo Fisher Scientific, 48–7319-41 Flow cytometry
CD3 and isotype Mouse 5 μl of antibody per test  Elabscience, E-AB-F1013J Flow cytometry
CD4 and isotype Mouse 5 μl of antibody per test Elabscience, E-AB-F1097C Flow cytometry
CD8 and isotype Mouse 5 μl of antibody per test Elabscience, E-AB-F1104D Flow cytometry
IFN-γ and isotype Mouse 5 μl of antibody per test Elabscience, E-AB-F1101E Flow cytometry