Fig. 4

Suppressive role of IL-18BP on osteoclastogenesis under RANKL stimulation. CD14⁺ monocytes from RA patients (N = 5) were cultured with RANKL (30 ng/mL) + M-CSF (25 ng/mL) without or with IL-18BP (10, 50, 100 ng/mL). A TRAP⁺ multinucleated cell count was measured. B The gene expression of TRAP, NFATc1, cathepsin K, and RANK from differentiated osteoclasts was measured by real-time quantitative PCR. Data were normalized to beta-actin and reported in relative expression units. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001