Fig. 7

CYT387 inhibits subcutaneous tumour growth in nude mice but has little impact on intracranial orthotopic tumour. A, B Nude mouse subcutaneous tumor volume was monitored after fed with CYT387 (100 mg/kg/day). C Tumor weight was measured after fed with solvent or CYT387 for 30 days. D The expressions of IKBKE, LATS-2, YAP1, p-YAP1, Axl, c-myc, cdk1, cyclinA₂ and cyclinB₁ were detected by immunohistochemistry. E Intracranial tumors were monitored by luminescence imaging on 7th, 14th and 21th day after orthotopic xenotransplantation. F Mouse weight was monitored every 2 days after orthotopic xenotransplantation. G The survival rate was presented after nude mice fed with solvent or CYT387. H The expressions of IKBKE, LATS-2, YAP1 and p-YAP1 were measured by immunohistochemistry. All immunohistochemical stainings were repeated at least three times