Fig. 6

IKBKE directly interacts with TEAD2 and YAP1 to regulate the Hippo pathway, accelerating TEAD2 and YAP1 translocation to nucleus. A The mRNA expression of TEAD2 and YAP1 were analysized by real-time RT-PCR after knocking down IKBKE. B IKBKE directly interacted with TEAD2 and YAP1 using endogenous co-IP. C Flag-IKBKE combined with HA-TEAD2 using exogenous co-IP. D Flag-IKBKE combined with HA-YAP1 using exogenous co-IP. E Inhibition of IKBKE suppressed YAP1 and TEAD2 translocation to nucleus by western blot (CE, cytoplasmic extraction; NE, nuclear extraction). F Mechanism of IKBKE influencing on the Hippo pathway. All experiments were repeated at least three times