Fig. 5

CYT387 activates the Hippo pathway via inhibiting IKBKE expression. A IKBKE and the Hippo pathway markers LATS-2, YAP1, p-YAP1, TEAD2 and its downstream factors Axl and c-myc were detected by western blot after knockdown of IKBKE in U87-MG and LN229. B IKBKE and the Hippo pathway markers LATS-2, YAP1, p-YAP1, TEAD2 and its downstream factors Axl and c-myc were detected by western blot after overexpression of IKBKE in U87-MG and LN229. C, D IKBKE, LATS-2, YAP1, p-YAP1, TEAD2, Axl and c-myc were measured by western blot after treatment with CYT387 in a dose-dependent and a time-dependent manner. E The expressions of IKBKE, LATS-2, YAP1, p-YAP1, TEAD2, Axl and c-myc were measured by western blot after overexpressing IKBKE and then treatment with CYT387 for 72 h. All experiments were repeated at least three times