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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: Overexpression of E3 ubiquitin ligase Cbl attenuates endothelial dysfunction in diabetes mellitus by inhibiting the JAK2/STAT4 signaling and Runx3-mediated H3K4me3

Fig. 6

Inhibitory role of Cbl in HUVEC apoptosis through Runx3-mediated JAK2/STAT4 signaling pathway. A Western blot analysis of expression of Cbl, JAK2, p-JAK2, STAT4, p-STAT4 and Runx3 in HUVECs upon treatment with HG + oe-NC, HG + oe-Cb1 + oe-NC, or HG + oe-Cb1 + oe-Runx3. B Quantification of NO content in HUVECs upon treatment with HG + oe-NC, HG + oe-Cbl + oe-NC, or HG + oe-Cbl + oe-Runx3 using Griess method. C eNOS protein level in HG-induced HUVECs determined by Western blot analysis. D Flow cytometry of apoptosis of HUVECs and corresponding quantification upon treatment with HG + oe-NC, HG + oe-Cbl + oe-NC, or HG + oe-Cb1 + oe-Runx3. * p < 0.05 vs. the HG + oe-NC group and # p < 0.05 vs. the HG + oe-Cbl + oe-NC group. Data were measurement data, and presented as mean ± standard deviation. Data among groups were analyzed by one-way ANOVA. Experiment was repeated three times independently

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Journal of Translational Medicine

ISSN: 1479-5876

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