Fig. 5

STAT4 facilitates apoptosis of HUVECs by Runx3 promotion. A Western blot analysis of STAT4 and Runx3 protein expression in HUVECs upon treatment with HG + sh-STAT4 + oe-NC, HG + sh-STAT4 + oe-Runx3, or controls. B Quantification of NO content in the culture supernatant of HUVECs upon treatment with HG + sh-STAT4 + oe-NC, HG + sh-STAT4 + oe-Runx3, or controls detected by Griess assay. C eNOS protein level in HG-induced HUVECs determined by Western blot analysis. D Flow cytometry of apoptosis of HUVECs and corresponding quantification upon treatment with HG + sh-STAT4 + oe-NC, HG + sh-STAT4 + oe-Runx3, or controls. * p < 0.05 vs. the HG + sh-NC + oe-NC group and # p < 0.05 vs. the HG + sh-STAT4 + oe-NC group. Data among groups were analyzed by one-way ANOVA. Experiment was repeated three times independently