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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: Overexpression of E3 ubiquitin ligase Cbl attenuates endothelial dysfunction in diabetes mellitus by inhibiting the JAK2/STAT4 signaling and Runx3-mediated H3K4me3

Fig. 3

Regulation of Cbl on JAK2 ubiquitination-affected JAK2/STAT4 pathway. A UbiBrowser database prediction on the E3 ubiquitin ligase of JAK2. B PPI network of Cbl, JAK2 and related genes by GeneMANIA; the larger circle of gene indicates higher core degree, and the smaller circle of gene indicates lower core degree. C Western blot analysis of protein expression of Cbl, JAK2, p-JAK2, STAT4 and p-STAT4 in HUVECs cultured in normal medium upon treatment of oe-NC, oe-Cbl or sh-Cb1. * p < 0.05 vs. the Con + oe-NC group and # p < 0.05 vs. the Con + sh-NC group. D Western blot analysis of JAK2 ubiquitination level of HUVECs upon treatment with MG132, oe-NC, or oe-Cbl. E, Western blot analysis of JAK2 degradation level in HUVECs cells after CHX treatment. * p < 0.05 vs. 0 h. Data were measurement data, and presented as mean ± standard deviation. The data between two groups was analyzed by unpaired t-test. Data among groups were analyzed by one-way ANOVA, followed by Tukey’s post hoc test. Experiment was repeated three times independently

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