Fig. 2

Effects of Cbl-mediated JAK2/STAT4 pathway on apoptosis of HUVECs. A Positive rate of p-JAK2 and p-STAT4 protein in rat aortic tissues from Con and DM rats (× 200). * p < 0.05 vs. the Con group, n = 6. B Western blot analysis of Cbl expression in HUVECs under Con or HG condition. * p < 0.05 vs. the Con group. C Western blot analysis of Cbl, JAK2, p-JAK2, p-STAT4 and STAT4 expression in Con or HG-treated HUVECs upon treatment with oe-NC or oe-Cbl. * p < 0.05 vs. the Con + oe-NC group and # p < 0.05 vs. the Con + oe-Cbl group. D Quantification of NO level in the culture supernatant of HUVECs upon treatment with Con + oe-NC, HG + oe-NC, and HG + oe-Cbl detected by Griess assay. * p < 0.05 vs. the Con + oe-NC group and # p < 0.05 vs. the Con + oe-Cbl group. E eNOS protein level in HG-induced HUVECs determined by Western blot analysis. F Flow cytometry of apoptosis of HUVECs and corresponding quantification upon treatment with Con + oe-NC, HG + oe-NC, and HG + oe-Cbl. * p < 0.05 vs. the Con + oe-NC group and # p < 0.05 vs. the HG + oe-NC group. Data were measurement data, and presented as mean ± standard deviation. The data between two groups was analyzed by unpaired t-test. Data among groups were analyzed by one-way ANOVA, followed by Tukey’s post hoc test. Cell experiment was repeated three times independently