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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: Overexpression of E3 ubiquitin ligase Cbl attenuates endothelial dysfunction in diabetes mellitus by inhibiting the JAK2/STAT4 signaling and Runx3-mediated H3K4me3

Fig. 1

Differential expression of Cbl in the microarray data, DM rat model, and HG-induced HUVEC model. A The downregulated genes in DM retrieved from GSE26168 microarray. B The downregulated genes in DM retrieved from GSE29221 microarray. C The intersection of top 100 significantly downregulated genes in the GSE26168 and GSE29221 datasets by Venn diagram; the two circles in the graph represent the downregulated genes in the two datasets respectively, and the overlapped part represents the intersection of two groups of data. D The boxplot of Cbl expression in GSE26168 dataset; the left blue box represents the normal samples, and the right red box represents the type 2 DM samples. E The boxplot of Cbl expression in GSE29221 dataset; the left blue box represents the normal samples, and the right red box represents the type 2 DM sample. F Quantification of fasting blood glucose and body weight of rat before and after modeling. G HE staining of aortic tissues in DM rats or control rats (× 200). H Quantification of the aortic relaxation response to ACH and SNP in DM rats or control rats. I IHC assay of positive-Cbl protein in DM rat aortic tissues and corresponding quantification (× 200); n = 6. J Morphology observation of HUVECs under Con or HG condition (× 400). K Western blot analysis of Cbl expression of HUVECs cells under Con or HG condition. * p < 0.05 vs. the Con group. Data were measurement data, and presented as mean ± standard deviation. The data between two groups was analyzed by unpaired t-test. Data at different time points among groups were compared by two-way ANOVA, followed by Tukey’s post hoc test. Cell experiment was repeated three times independently

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