An update on extra-oral bitter taste receptors

Table 2 Expression and distribution of TAS2Rs in different normal mouse tissue types

System		Receptor type																																	Methods	Refs.
System		102	103	104	105	106	107	108	109	110	113	114	116	117	118	119	120	121	122	123	124	126	129	130	131	134	135	136	137	138	139	140	143	144	Methods	Refs.
Respiratory system	SCCs										+																			+					ICC, ISH	[11]
Immune system	Thymus				+			+																	+										IHC, ISH, qRT-PCR	[120, 122]
Cardio-vascular system	Heart														+							+					+						+		ISH, qRT-PCR	[202, 203]
	Arteries												+																				+		FC, IFC, RT-PCR	[70]
Reproductive system	Testis and sperms	All 35 mTAS2Rs																																	ICC, IHC, ISH, RT-PCR, qRT-PCR	[134, 135]
Digestive system	Tuft cells														+																				RNAscope hybridisation and imaging	[139]
	Paneth cells																								+										IHC, ISH, RT-PCR	[147]
	Goblet cells																								+										IHC, ISH, RT-PCR	[147]
	EECs	+		+	+	+	+	+		+	+	+	+	+		+		+	+	+	+	+	+	+		+	+	+	+	+	+	+	+	+	ICC, RT-PCR	[159, 164]
	Gastrointestinal smooth muscle cells							+																					+						RT-PCR	[175]
Excretory system	Kidney	+								+	+		+		+							+	+	+	+		+	+	+	+	+	+	+	+	IHC, IFC, RT-PCR, qRT-PCR, WB	[209,210,211]
	DMS	+	+					+	+		+	+	+	+	+	+	+		+			+		+	+		+		+			+	+	+	qRT-PCR	[73]
Nervous system													+																						IHC, ISH, RT-PCR	[116, 191]
Adipose tissue										+	+				+	+						+				+	+		+	+		+	+	+	RT-PCR	[216]

*DMS detrusor smooth muscle, EECs enteroendocrine cells, FC flow cytometry, ICC immunocytochemistry, IFC immunofluorescence, IHC immunohistochemistry, ISH in situ hybridisation, RT-PCR reverse transcription-polymerase chain reaction, qRT-PCR quantitative reverse transcription-polymerase chain reaction, SCCs solitary chemosensory cells, WB Western blotting

ISSN: 1479-5876