Fig. 4

SERPINA3 inhibits cell apoptosis while promoting autophagy and aerobic glycolysis in PCa cells. A, B The knockdown efficiency of sh-SERPINA3-1/2/3 and overexpression efficiency of pcDNA3.1-SERPINA3 were examined through RT-qPCR. C, D An analysis of annexin V staining by flow cytometry was utilized to test apoptosis rate of PCa cells when SERPINA3 was down-regulated or overexpressed. E, F Caspase3 Activity Assay Kit was applied to examine Caspase3 activity when SERPINA3 was reduced or overexpressed in PCa cells. G, H Caspase9 activity was measured by means of Caspase9 Activity Assay Kit in PCa cells with SERPINA3 knockdown or elevation. I, J Western blot analysis was carried out to examine the expression of LC3-I, LC3-II and p62 when SERPINA3 was diminished in PCa cells. K, L The expression of LC3-I, LC3-II and p62 was analyzed by western blot assay when PCa cells were transfected with pcDNA3.1-SERPINA3. M, N The glucose consumption in PCa cells was tested when SERPINA3 was down-regulated or up-regulated. O, P Lactate content was quantified in PCa cells with SERPINA3 depletion or augment. Q, R PK activity was measured after PCa cells were co-cultured with sh-SERPINA3-1/2 or pcDNA3.1-SERPINA3. S, T ECAR/OCR value in PCa cells were examined upon SERPINA3 decline or overexpression. (sh short hairpin, NC negative control). ^**P < 0.01