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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: CircSERPINA3 regulates SERPINA3-mediated apoptosis, autophagy and aerobic glycolysis of prostate cancer cells by competitively binding to MiR-653-5p and recruiting BUD13

Fig. 1

circSERPINA3 inhibits cell apoptosis while promoting autophagy and aerobic glycolysis in PCa cells. A RT-qPCR was utilized to examine circSERPINA3 expression in normal WPMY-1 cell line and PCa cells (PC-3, DU145 and VCaP). B The expression level of circSERPINA3 after RNase R treatment was examined by RT-qPCR. C AGE-PCR was used to verify the closed-loop structure of circSERPINA3 in PC-3 cells. D The knockdown efficiency of sh-circSERPINA3-1/2/3 in PC-3 cells was examined via RT-qPCR. E The overexpression efficiency of pcDNA3.1-circSERPINA3 in PC-3 cells was tested by means of RT-qPCR. F The expression of circSERPINA3 was detected via RT-qPCR after DU145 cells were transfected with sh-circSERPINA3-1/2/3. G The expression of circSERPINA3 was quantified via RT-qPCR after DU145 cells were transfected with pcDNA3.1-circSERPINA3. H, I An analysis of annexin V staining by flow cytometry was applied to test the apoptosis rate of PCa cells upon circSERPINA3 depletion or overexpression. J, K Caspase3 Activity Assay Kit was applied to examine Caspase3 activity when circSERPINA3 was cut down in PCa cells. L, M In response to circSERPINA3 knockdown or augment, Caspase9 activity in PCa cells was detected by Caspase9 Activity Assay Kit. N, O Western blot analysis was performed to examine the protein levels of LC3-I, LC3-II and p62 in PCa cells after circSERPINA3 down-regulation. P, Q The protein levels of LC3-I, LC3-II and p62 were detected by western blot after circSERPINA3 augment in PCa cells. R, S Glucose consumption in PCa cells was examined after circSERPINA3 down-regulation or overexpression. T, U Lactate content was detected in PCa cells transfected with sh-circSERPINA3-1/2 or pcDNA3.1-circSERPINA3. V, W PK activity was examined in PCa cells with circSERPINA3 knockdown or up-regulation. X, Y ECAR/OCR value in PCa cells was tested under the condition of circSERPINA3 depletion or overexpression. (cDNA complementary DNA, gDNA genomic DNA, sh short hairpin, NC negative control). *Pā€‰<ā€‰0.05, **Pā€‰<ā€‰0.01

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