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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: A direct negative feedback loop of miR-4721/FOXA1/Nanog promotes nasopharyngeal cell stem cell enrichment and metastasis

Fig. 5

miR-4721 regulates FOXA1-modulated CSC-associated genes and EMT markers. A EMSA assay of FOXA1 binding to Nanog promoter in NPC cells. B CHIP experiment shows FOXA1 binds miR-4721 promoter region. C Relative luciferase activity of the indicated promoter vectors HONE1 and SUNE1 cells transfected with FOXA1 plasmids. D Immunofluorescence staining of Nanog after transfection with NC, ectopic FOXA1, and miR-4721 as indicated. Scale bar, 100 px. B miR-4721 modulates CSC-associated genes and EMT markers via the FOXA1/NANOG pathway in HONE1 and SUNE1. E-cadherin, ZEB2, and Snail with NC, ectopic FOXA1, and/or miR-4721 as indicated in NPC cells by Western blot analysis

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