Fig. 3

miR-4721 directly regulates FOXA1. A The putative binding sequences of miR-4721 in the 3'UTR of FOXA1. Mutation in the complementary site that binds to the seed region of miR-4721. B QPCR is used to detect the expression of FOXA1 in miR-4721-overexpressing NPC cells, normalized to U6. C Western blot analysis of FOXA1 in miR-4721-overexpressing NPC cells, with β-actin as a loading control. D Immunofluorescence staining of FOXA1 after transfection with NC, ectopic FOXA1, and miR-4721 as indicated. Scale bar, 100 px. (E) miR-4721 directly targeting the FOXA1 3ʹUTR, as determined by luciferase reporter assay. *P < 0.05