Fig. 4

Bufalin suppresses TME-mediated angiogenesis by directly affecting vascular endothelial cells. Tube formation (a), migration (b), and adhesion (c) of HUVECs treated with TME-CMs and TME + BU-CMs for 24 h. d–f WB showing the protein expression of p-STAT3 and STAT3 in HUVECs treated with different TME-CMs and TME + BU-CMs, membranes were stripped and re-probed with total β-actin as a control. N.S. indicates no significant difference between the two groups, P > 0.05. Data are shown as mean s.e.m. BU, bufalin