Fig. 1

Elevated expression of UBE2T in most lung cancer cells. Western blot was used to detect UBE2T. A, B Comparison of UBE2T expression in normal lung bronchial epithelial and lung cancer cells. C, D UBE2T was overexpressed in A549 cells infected with a lentiviral vector carrying UBE2T cDNA. Differential gene silencing efficiency in A549 cells by three lentivirus-mediated short-hairpin RNAs designed against UBE2T (shUBE2T). E, F Establishment and validation of stable A549 cells with knockdown of UBE2T or overexpressing UBE2T. Western blot was conducted at least three times to validate the stable cells. GAPDH was used as internal controls. G UBE2T overexpression promoted the proliferation of A549 cells. H Silencing of UBE2T showed inhibitory effects on cell proliferation. All experiments were repeated at least three times. Protein bands were quantified using Image J. *P < 0.05, **P < 0.01, ***P < 0.001