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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Long non-coding RNA NORAD promotes pancreatic cancer stem cell proliferation and self-renewal by blocking microRNA-202-5p-mediated ANP32E inhibition

Fig. 4

NORAD competitively binds to miR-202-5p and reduces the expression of miR-202-5p in PCSCs. A Distribution of NORAD in PC cells (400×); B Specific binding sites of NORAD and miR-202-5p; C Targeting relationship between miR-202-5p and NORAD measured by dual-luciferase reporter assay (* p < 0.05 vs. cells co-transfected with NORAD-WT-Luc and miR-202-5p mimic-NC, NORAD-MUT-Luc and miR-202-5p mimic, NORAD-MUT-Luc and miR-202-5p mimic-NC); D NORAD targeted miR-202-5p verified by RIP assay (* p < 0.05 vs. IgG pulldown samples); E NORAD targeted miR-202-5p verified by RNA pull-down assay (* p < 0.05 vs. Bio-NC probe labeled samples); F NORAD and miR-202-5p expression as well as the mRNA expression of ANP32E in PCSCs measured by RT-qPCR (* p < 0.05 vs. cells without treatment). Measurement data were expressed as mean ± standard derivation. Data between two groups were compared by unpaired t-test, and data among multiple groups were analyzed by one-way analysis of variance with Tukey’s post hoc test. The experiment was repeated three times

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