Fig. 5

MiR-525-5p targets VMA21 and inhibits VMA21 expression in LUAD cells. A The interaction between miR-525-5p and VMA21 was assessed by dual luciferase assay. B Western blotting for the protein level of VMA21 in A549 cells transfected with miR-525-5p inhibitor or mimics. C RT-qPCR for determining VMA21 expression in LUAD and normal adjacent tissues. D The VMA21 level in multiple LUAD cells and normal human bronchial epithelial cells was detected by RT-qPCR. E The correlation between VMA21 and miR-525-5p was analyzed. All data are expressed as the means ± standard deviation from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 versus the indicated group