Fig. 4

Circ_0001361 facilitates the progression of LUAD cells via sponging miR-525-5p to increase VMA21 expression. RT-qPCR (A) and Western blotting (B) for the expression of VMA21 in A549 and PC9 cells from different groups. The proliferation of A549 and PC9 cells was determined by MTT assay (C) and colony formation assay (D). E Wound scratch assay for the migration of A549 and PC9 cells with various treatments. F The invasive ability of A549 and PC9 cells was detected by Transwell assay. All data are expressed as the means ± standard deviation from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 versus the indicated group