Fig. 2

Transcriptomic analysis based on organoids and tissues identified different mechanisms in LUAD and LUSC. a Venn diagram showed the intersection of pathways enriched in LUAD (n = 58) and LUSC (n = 49) tissues compared with the matched normal tissues. b Venn diagram showed the intersection of pathways enrichment between LUAD (n = 6) and LUSC (n = 2) organoids, which were also enriched in the lung cancer tissues compared with normal tissues. c-e Venn diagram showed the overlapping pathways upregulated in NSCLC tissues but with no difference between LUAD (n = 6) and LUSC (n = 2) organoids (c), 18 signaling pathways downregulated in NSCLC tissues but with no difference between LUAD (n = 6) and LUSC (n = 2) organoids (d), and 42 signaling pathways downregulated in NSCLC tissues, which were further downregulated in LUAD organoids compared with LUSC organoids (e). up: upregulated genes; down: downregulated genes