Fig. 5
From: Metformin attenuates silica-induced pulmonary fibrosis via AMPK signaling
Metformin inhibits SiO2-induced pulmonary macrophage inflammatory response. A, B, D and E qRT-PCR detection of TNF-α (A, D) and IL-1β (B, E) mRNA expression in THP-1 cells from different concentration SiO2 and different time treatment, with *p < 0.05 and **p < 0.01 vs. control group. C Western blots of TNF-α and IL-1β in THP-1 cells after incubated with SiO2. F, G qRT-PCR detection of TNF-α (F) and IL-1β (G) mRNA expression in THP-1 cells after silica and metformin co-treatment for 12 h, with **p < 0.01 vs. control and #p < 0.05 vs. the silica group. H Western blots of TNF-α and IL-1β in THP-1 cells after incubated with SiO2 and metformin for 12 h