Fig. 3

Hierarchical clustering and relative levels the of TOP 50 lipids. The scaled concentration value of each lipid from the TOP 50 identified by MetaboAnalyst are projected on level heatmap for each group. The color legend identifies in red the lipids in high level and blue the lipids at lower concentration (A). Relative changes in females (top) and males (bottom) between ME/CFS patients and their respective controls are represented on the Volcano plot (B). Each dot represents one individual lipid from the TOP 50 identified by the clustering and the red dots indicate the significant changes relative to controls with p-value threshold set at 0.05 (horizontal dotted line). The vertical dotted lines represent the arbitrary fold-change (FC) cut-off of ± 1.2