Fig. 5

Iron deprivation drives M2 polarization of macrophage through induction of HIF-1α. A, B The protein expression of HIF-1α in PMs with indicated treatment by Western blot. C The protein expression of HIF-1α in PMs co-cultured with indicated cells or DMEM alone by Western blot. D–F The mRNA expression of Arg1, Vegfa, and Pd-l1 by qPCR in PMs with either Hif-1α knockdown or its control counterpart upon indicated treatment. The results were shown as 2^−ΔΔCt and were expressed as relative fold changes normalized to the controls. G, H Cell surface expression of CD206 and PD-L1 by FACS in PMs with either Hif-1α knockdown or its control counterpart upon indicated treatment. The results were shown as relative fold changes in MFI of CD206 and PD-L1 normalized to the controls. I The protein expression of HIF-1α in PMs treated as described. J The mRNA expression of Arg1, Vegfa, and Pd-l1 was determined by qPCR in PMs treated as described. The fold changes in expression level relative to control were expressed as 2^−ΔΔCt. All data are representative of three independent experiments and presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001