Fig. 3

Iron deprivation drives the immunosuppressive function of macrophages. A, B CFSE-labeled murine spleen CD4⁺ and CD8⁺ T cells were co-cultured with BMDMs and PMs treated as described at a ratio of 1:2 for 3 days, and the fluorescence of CSFE was measured by FACS. The proliferation rate of T cells was determined as the proportion of cells with reduced CFSE intensity due to cell division. All data are representative of three independent experiments and presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001