Fig. 7

Dex ameliorates intestinal I/R injury in rats through the SIRT3-mediated PINK1/HDAC3/p53 pathway. A Intestinal histopathological changes in rats. B Intestinal damage assessed by Chiu’s score. C Intestinal W/D ratio. D Level of MDA in intestinal tissues of rats. E SOD activity in intestinal tissues of rats. F Serum expression of TNF-α in rat tissues. G Serum expression of IL-6 in rat tissues. H The positive expression of GFAP and S-100β in the intestinal tissue of rats evaluated by IHC. I Western blot analysis for measurement of HDAC3 phosphorylation level and protein expression of GFAP, S-100β, SIRT3, PINK1, Bax, Bcl-2, Atg5, p62, p53, HDAC3, caspase-3, and cleaved caspase-3 as well as LC3-II/I ratio in intestinal tissues of rats. J The apoptosis of EGCs in intestinal tissues of rats examined by TUNEL. *p < 0.05 vs. sham-operated rats; ^#p < 0.05 vs. I/R-exposed rats; ^&p < 0.05 vs. I/R-exposed rats with Dex treatment. Measurement data were expressed as mean ± standard deviation. Comparison among multiple groups was conducted by one-way ANOVA with Tukey’s post hoc test. N = 10