Fig. 6

Dex ameliorates TNF-α- and IFN-γ-stimulated mitochondrial damage and apoptosis in EGCs by the SIRT3-mediated PINK1/HDAC3/p53 pathway. A Examination of MMP changes in EGCs. B The apoptosis of EGCs assessed by flow cytometry. C Western blot analysis for measurement of protein expression of SIRT3, PINK1, Bax, Bcl-2, Atg5, p62, p53, caspase-3, and cleaved caspase-3 along with LC3-II/I ratio in the EGCs. D Apoptosis of EGCs was detected by Hoechst33342 staining and observed in a fluorescence microscope. *p < 0.05 vs. EGCs treated with 50 ng/mL of TNF-α and 100 ng/mL of IFN-γ; ^#p < 0.05 vs. EGCs treated with 50 ng/mL of TNF-α, 100 ng/mL of IFN-γ and 1 μM of Dex. Measurement data were expressed as mean ± standard deviation. Comparison among multiple groups was conducted by one-way ANOVA with Tukey’s post hoc test. The cell experiment was repeated three times