Fig. 4

PINK1 reduces p53 expression by promoting the phosphorylation of HDAC3. A Binding between HDAC3 and p53 in EGCs verified by Co-IP. B HDAC3 phosphorylation level and protein expression of PINK1, HDAC3, and p53 in EGCs transfected with sh-PINK1 determined by Western blot analysis. C Interaction among PINK1, HDAC3, and p53 in EGCs transfected with sh-PINK1 identified by Co-IP. D Western blot analysis for determination of protein expression of HDAC3 and p53 in EGCs with RGFP966 treatment. E The acetylation level of p53 assessed by Co-IP. F Binding between HDAC3 and PINK1 in EGCs verified by Co-IP. G HDAC3 phosphorylation level and protein expression of PINK1, HDAC3, and p53 in EGCs transfected with sh-PINK1 measured through Western blot analysis. *p < 0.05 vs. EGCs transfected with sh-NC, EGCs treated with 50 ng/mL of TNF-α and 100 ng/mL of IFN-γ) or TNF-α and IFN-γ -exposed EGCs transfected with sh-PINK1. Measurement data were expressed as mean ± standard deviation. Comparison among multiple groups was conducted by one-way ANOVA with Tukey’s post hoc test. p < 0.05 was indicative of statistical significance. The cell experiment was repeated three times