Fig. 3

Dex restrains TNF-α- and IFN-γ-stimulated mitochondrial damage and apoptosis in EGCs through the SIRT3-mediated PINK1-related pathway. A Detection of MMP changes in EGCs. B The apoptosis of EGCs examined by flow cytometry. C Protein expression of SIRT3, PINK1, Bax, Bcl-2, Atg5, p62, p53, caspase-3, and cleaved caspase-3 along with LC3-II/I ratio in the EGCs measured by Western blot analysis. *p < 0.05 vs. the Mod group (EGCs treated with 50 ng/mL of TNF-α and 100 ng/mL of IFN-γ); ^#p < 0.05 vs. EGCs treated with 50 ng/mL of TNF-α, 100 ng/mL of IFN-γ and 1 μM of Dex. Measurement data were expressed as mean ± standard deviation. Comparison among multiple groups was conducted by one-way ANOVA with Tukey’s post hoc test. The cell experiment was repeated three times