Fig. 5

GAS5 promotes AAA progression through regulation of the EZH2/RIG-I axis. A Experiment flow chart. B The expression of GAS5, EZH2 and RIG-I in aortic tissues of control or AAA mice treated with sh-EZH2, sh-GAS5, oe-RIG-I, sh-NC and oe-NC detected by RT-qPCR. C Western blot analysis of EZH2 and RIG-I proteins in mouse aortic tissues. D The maximum abdominal aorta diameter of mice. E Representative TUNEL staining image of Cell apoptosis in mouse aortic tissues of control or AAA mice treated with sh-EZH2, sh-GAS5, oe-RIG-I, sh-NC and oe-NC (400 ×). F Quantification of TUNEL positive ratio of cell apoptosis in mouse aortic tissues. G Western blot analysis of cleaved caspase-3, Bcl-2 and Bax proteins in mouse aortic tissues. ^@p < 0.05 vs. the control group. *p < 0.05 vs. the AAA + oe-NC + sh-NC group; ^#p < 0.05 vs. the AAA + sh-GAS5 + oe-NC + sh-NC group. Data in A–G were compared using one-way ANOVA with Tukey’s post-hoc tests. n = 6 for mice following each treatment