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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: Heparin-binding growth factor (HDGF) drives radioresistance in breast cancer by activating the STAT3 signaling pathway

Fig. 6

Inhibition of breast cancer radioresistance by HDGF depletion combined with STAT3 activity inhibition. A, C, D Effects of Stattic (20 μmol/L) with or without HDGF knockdown on STAT3 phosphorylation level (A), cell proliferation (C), and surviving fractions (D) after irradiation. B Quantification of protein in (A). E Representative bioluminescence images of Stattic (20 μmol/L) or Stattic (20 μmol/L) combined with HDGF knockdown -transfected MDA-MB-231 cells injected into the mice mammary gland fat pads. Mice were imaged 4 weeks after transplantation. Data were from two independent experiments with 5 mice per group with similar results. F Quantification of bioluminescence activity in (E). G the working model showing that RXRα binds with HDGF promoter and suppresses HDGF transcriptional activity. HDGF could associate with TKT and STAT3, which promotes STAT3 phosphorylation and transcriptional activity, thereby exerts tumor radioresistance in breast cancer. As shown in B, C, D, and F Error bars ± S.D. *P < 0.05. ***P < 0.001. Data are representative of three independent experiments

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