Fig. 3

Negative modulation of HDGF by RXRα. A Schematic diagram of the putative RXRα binding site in HDGF promoter. B, C RXRα knockdown inhibited HDGF mRNA (B) and protein (C) in MCF7 and MDA-MB-231 cells. D Quantification of HDGF protein in (C). E ChIP-qPCR assay of RXRα binding with the HDGF promoter in MDA-MB-231 cells. F Luciferase assay of RXRα inhibiting HDGF promoter activity in MDA-MB-231 cells. G Effects of 9-cis-retinoic acid (20umol/L) with or without HDGF overexpression co-transfection into MDA-MB-231 cells. H Quantification of HDGF protein in (G). I–K HDGF rescued 9-cis-retinoic acid (20umol/L)-inhibited cell proliferation (I, J) and surviving fractions (K) after irradiation. As shown in B, D, E, F, G, H, I, J, and K, Error bars ± S.D. *P < 0.05. **P < 0.01. ***P < 0.001. Data are representative of three independent experiments