Fig. 2

HDGF-enhanced radioresistance of breast cancer cells. A Western blot analysis of HDGF protein levels in HDGF knockdown breast cancer cells. B Quantification of HDGF protein in (A). C, D NPC cells downregulating HDGF show significantly lower surviving fractions compared to breast cancer cells post-irradiation at 2, 4, 6, 8, and 10 Gy after 2 weeks. E, F, Breast cancer cells downregulating HDGF show slower proliferation after 4 Gy irradiation at 24, 48, 72, 96, and 120 h. G, Representative xenograft tumors of HDGF knockdown-inhibited tumor size in MDA-MB-231/IR cells. H, Quantification of tumor size in (F). As shown in B, C, D, E, F, and H, Error bars ± S.D. *P < 0.05. ***P < 0.001. Data are representative of three independent experiments