Fig. 5

miR-525-5p downregulates FGF11 expression and inhibits NSCLC cell proliferation and migration. A Bioinformatic search of miRNAs targeting FGF11 using miRDB, Starbase and Targetscan databases. B qPCR analysis of FGF11 mRNA expression in A549 and NCI-H460 cells in the presence of microRNA mimics. c Dual-luciferase reporter assay of the effect of miR-525-5p mimic on FGF11 3′UTR. D western blot detected the expression of FGF11 after transfected with miR-525-5p. E qPCR analysis of the FGF11 mRNA level in NSCLC tumor tissues and normal tissues. F The correlation of miR-525-5p level and FGF11 level was examined by Pearson’s correlation analysis. G CCK-8 proliferation assay of A549 and NCI-H460 cells transfected with miR-525-5p and/or FGF11. H Colony formation assay in A549 and NCI-H460 cells transfected with miR-525-5p and/or FGF11. I Wound healing assay in A549 and NCI-H460 cells transfected with miR-525-5p and/or FGF11. J Transwell migration assay in A549 and NCI-H460 cells transfected with miR-525-5p and/or FGF11. The results are represented as mean ± standard derivation. Data are summary of 3 independent experiments. *P < 0.05, ** P < 0.01, ***P < 0.005 vs. sh-NC or empty vector-transfected cells