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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: E3 ubiquitin ligase TRIM29 promotes pancreatic cancer growth and progression via stabilizing Yes-associated protein 1

Fig. 6

TRIM29 stabilized YAP1 by regulating its ubiquitination in pancreatic cancer cells. A co-IP results showed that TRIM29 and YAP1 interacted directly. B The expression level of YAP1 increased with time in pancreatic cancer (PC) cells treated with proteasome inhibitor MG132. C and D TRIM29 did not affect YAP1 expression level, as assessed in PC cells transfected with shTRIM29/P-TRIM29 plasmid and treated with MG132. E and F Detection of YAP1 degradation using anti-HA and anti-Flag antibodies in BxPC-3 and SW1990 cells transfected with the HA-YAP1 expression plasmid, with or without the Flag-TRIM29 plasmid, and treated with cycloheximide for the indicated times. G Lysates from PC cells transduced with shTRIM29/P-TRIM29 were treated with MG132 (15 µM) for 4 h, collected, immunoprecipitated using an anti-ubiquitin (Ub) antibody, and then immunoblotted using a YAP1 antibody

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