Fig. 8

BCLAF1 phosphorylation at Ser290 protects cells from IR-induced cell apoptosis. a The shNC and sh2 stable cells were treated with 15 Gy IR for 2 h, and the percentage of cell apoptosis was determined using flow cytometry. Data are shown as mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 and ***p < 0.001 vs. shNC + 15 Gy. b Moreover, HGC and AGS cells stably overexpressing WT, S290D, S290A forms of BCLAF1 were treated with 15 Gy IR for 2 h, and the percentage of cell apoptosis was determined using flow cytometry. Data are shown as mean ± SD of three independent experiments. *p < 0.05 and **p < 0.01 vs. WT + 15 Gy, ^#p < 0.05 vs. EV + 15 Gy based on Student's t-test