Fig. 5

BCLAF1 phosphorylation at Ser290 facilitates cell proliferation. a MTT assay of HGC and AGS cells overexpressing BCLAF1-WT (WT), mimic BCLAF1-pSer290 (S290D), nonphosphorylatable Ser290 (S290A) or empty vector (EV). b Colony formation assay was performed to investigate colony formation ability in HGC and AGS cells overexpressing WT, S290D, S290A forms of BCLAF1. c Quantitative results of colony formation analyzed with Image J. d EdU incorporation assay was performed using a fluorescence method in cells transfected with WT, S290D, S290A forms of BCLAF1. Scale bars, 100 µm. e For each group, three different fields were randomly chosen and EdU positive cells were counted with Image J. Data are shown as mean ± SD. *p < 0.05, **p < 0.01 and ***p < 0.001 vs. WT. ^#p < 0.05, ^##p < 0.01 vs. EV based on Student's t-test