Fig. 2

Phosphorylation of BCLAF1 Ser290 is significantly increased in gastric cancer. a Total counts of phosphorylated residues in BCLAF1. **p < 0.01 vs. Non-tumor based on Student's t-test. b MS/MS spectrum of pSer290-containing peptide, YSPSQN(pS290)PIHHIPSR in HGC gastric cancer cells. c Dot blotting of the phosphorylated (pSer290) and unmodified (Ser290) peptides with anti-pBCLAF1 (Ser290) antibody. d Western blotting of the total proteins from HGC cells overexpressing WT, S290A and S290D forms of BCLAF. 177 kD, GFP-tagged BCLAF; 150 kD, endogenous BCLAF