Skip to main content
Fig. 5 | Journal of Translational Medicine

Fig. 5

From: Genetic alterations and functional networks of m6A RNA methylation regulators in pancreatic cancer based on data mining

Fig. 5

Validation of the function of METTL14 in tumor proliferation. AF Real-time quantitative polymerase chain reaction (RT-qPCR) and western blotting (WB) results of METTL14 knockdown and overexpression in PC cell lines (BxPC-3 and SW1990) and HPDE6-C7 cell. GI IncuCyte live cell imaging system results of growth curve in BxPC-3, SW1990 and HPDE6-C7 cells transfected with control, shRNAs and overexpression plasmids (0–96 h after treatment). JL CCK8 analysis of BxPC-3, SW1990 and HPDE6-C7 cells transfected with control, shRNAs and overexpression plasmids (48 h after treatment). NC represents negative control group; shMETTL14-09, shMETTL14-10, and shMETTL14-11 represent cells transfected with corresponding shRNAs groups; METTL14-OE represents cells transfected with METTL14 overexpression plasmid group; ns represents not significant; * represents P < 0.05; ** represents P < 0.01; and *** represents P < 0.001

Back to article page