Fig. 6

Effect of miR-92a-3p in skeletal muscle cells. A Skeletal muscle biopsy samples were obtained from pregnant women at the time of delivery and primary skeletal muscle cell cultures were developed. The skeletal muscle cells were transfected with miR-92a-3p mimic and transfection efficiency and miR-92-3p activity was analysed by SOCS5-3′ UTR luciferase assay B miR-92a-3p expression in transfected skeletal muscle cells measured by real-time PCR. miRNA expression is expressed as log2 values of foldchange. C Dual luciferase assay of SOCS5 3’UTR plasmid and control plasmid in skeletal muscle cells transfected with miR-92a-3p and negative control miRNA D JAK/STAT PCR array after transfection of skeletal muscle cells with miR-92a-3p and control scramble. Volcano plot depicting the genes upregulated and downregulated in cells transfected with miR-92a-3p compared to control miRNA (E) Gene ontology analysis of SOCS2 and NOS2 target proteins Poly(ADP-ribose) glycohydrolase (PARG), C–C motif chemokine 9 (Ccl9), T cell specific GTPase 1 and 2 (Tgtp1&2), Cluster of Differentiation 200 (CD200), Leukocyte immunoglobulin-like receptor subfamily B 4 (LILRB4), Interferon alpha and Chemokine (C–X–C motif) ligand 9 (CXCL9) and pathways associated with diabetes and hyperglycaemia. (F) Insulin-stimulated glucose uptake in skeletal muscle cells transfected with miR-92a-3p and control miRNA and non-transfected cells