Fig. 3From: Extracellular vesicle-associated miRNAs are an adaptive response to gestational diabetes mellitusValidation of GDM-associated miRNAs within EVs in a longitudinal independent cohort. The expression of selected miRNAs in EVs was determined in EVs isolated from NGT and GDM pregnancies across gestation. Left: data is presented as individual values of the same women at three times during gestation. Right: Linear mixed modelling analysis of real-time PCR data for candidate miRNAs for NGT and GDM pregnancies across gestation. Linear mixed modelling (lme4 package in R) was used to analyze the normalized CT values generated from real-time PCR data. The miRNAs were normalized to RNU6B using the ∆CT method, and the normalized values raised to power 2 for easier interpretation of results. Within the panels, the color gray is for normal pregnancies whilst the color black is for GDM pregnanciesBack to article page