Fig. 2

Radiation-induced long non-coding RNA expression profiles in mouse heart tissue. Whole genome microarray data was filtered to include only probes that correspond to transcripts of lncRNAs. A linear model was fit to each lncRNA probe to assess differential expression of irradiated compared to control samples using criteria of |log₂FC| > 2 and B-H p-value < 0.05. A Heatmap displays expression patterns, represented by z-score, of all differentially expressed lncRNAs across all doses and controls. B Venn diagram shows dose distribution and overlap of differentially expressed lncRNAs across all doses. C The table shows the number of down- versus up-regulated lncRNAs at each dose. D Examples of significant linearly up- and down-regulated lncRNAs are shown to display the dose response of lncRNAs to radiation in heart tissue samples. E RT-qPCR validation was performed on significantly up-regulated lncRNAs that were previously reported in the blood [44]